Amounts given to animals and healthy people
TB-500 Dosage: What each test gave
These amounts describe research, not care. People received the whole protein. No one has measured how long the short piece stays in human blood.
TB-500 Dosage: What animals and people received
TB-500 dosage claims often begin with animal research. Those animals received whole thymosin beta-4. One human safety test, called Phase 1, also used thymosin beta-4. The amounts below aren't directions for you. No study gives a human amount for the fragment.
Mouse and rat tests used a wide spread. Heart and brain work used about 6–12 milligrams per kilogram of the animal's weight. That means researchers set the amount by animal weight. In one rat stroke test, 2 and 12 milligrams for each kilogram helped. The 18 milligrams for each kilogram amount didn't [13]. A six-month test gave weak mice 150 micrograms twice a week [4]. Researchers used a shot into the open space inside the belly. A microgram is one millionth of a gram. A dish test used 10 trillionths of a gram [3]. Skin cells moved, but no whole wound was present.
Phase 1 gave 42, 140, 420, and 1260 mg through a vein. The healthy people got one amount first. Then they received it each day for 14 days [6]. No serious harm was linked to those amounts. Higher amounts led to more thymosin beta-4 in the blood.
Rodent tests often used a shot inside the belly. The Phase 1 human study used a vein. Eye and skin work also used thymosin beta-4. Researchers called that study form RGN-259 [3]. Sports users share their own plans. No sound study in people has checked those plans.
These are research notes, not your dose.
Half-Life: Human blood tests give no answer
Researchers don't know the human TB-500 half-life. Half-life means the time until half an amount remains. No sound human figure exists.
Phase 1 measured the whole protein in blood. Blood levels rose across amounts from 42–1260 mg [6]. The study didn't measure how fast TB-500 left the blood. Sports labs have found TB-500 and pieces formed after it broke apart. They checked horse and human samples. That only shows some trace lasted long enough to find. It doesn't reveal when half was gone.
Sellers often provide the fragment as a dry powder. The short piece can break apart with poor handling. An unchecked vial may also contain the wrong amount, another substance, or dirt. None of those facts gives you a safe way to mix or use it.
A human blood study is needed before you can trust any time claim.

What failed when stroke rats got more
One stroke test compared several amounts in rats. The animals improved at 2 and 12 milligrams per kilogram of the animal's weight. They didn't improve at 18 milligrams for each kilogram [13]. The amount was tied to each rat's weight.
The largest amount failed.
This was one animal test after strokes. It doesn't set an amount for a person. It also can't tell a doctor what would be safe. Giving more did not bring more help.
Your care can't be set from this rat result.